A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Abstract Background Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of and play vital roles in diverse physiological processes regulating plant growth development. Results CaCRK5 transcripts were induced pepper upon the infection Ralstonia solanacearum treatment with salicylic acid. The fusions between green fluorescence protein targeted to plasma membrane. Suppression via virus-induced gene silencing (VIGS) made plants significantly susceptible R. infection, which was accompanied decreased expression defense related genes CaPR1 , CaSAR8.2 CaDEF1 CaACO1 . Overexpression increased resistance against Nicotiana benthamiana Furthermore, electrophoretic mobility shift assay chromatin immunoprecipitation coupled quantitative real-time PCR analysis revealed that homeodomain zipper I CaHDZ27 can active through directly binding its promoter. Yeast two-hybrid bimolecular complementation (BiFC) analyses suggested heterodimerized homologous member CaCRK6 on Conclusions Our data played positive role immune responses pepper.